Real-time quantitative PCR for the detection of minimal residual disease in acute lymphoblastic leukemia using junctional region specific TaqMan probes

Detection of minimal residual disease in acute lymphoblastic leukemia using immunoglobulin hypervariable region specific oligonucleotide probes.

To develop a sensitive and specific assay for minimal residual disease in acute lymphoblastic leukemia (ALL), we exploited the enormous diversity of genomic sequences created by immune receptor gene rearrangements. To isolate clone-specific sequences, we first synthesized oligonucleotides that match conserved variable (VH) and joining (JH) sequences flanking the third hypervariable region (HVR3...

Evaluation of Relationship between Minimal Residual Disease (MRD) and Relapse in Childhood Acute Lymphoblastic Leukemia (ALL) Using Quantitative Fluorescent PCR

Production of Patient Specific Probes for the Detection of Minimal Residual Disease in Acute Lymphoblastic Leukemia

Twenty-two patients with childhood ALL were analyzed for the configuration of their Ig (innunoglobulin) and TcR-6 (T cell receptor delta) genes by Southern blot analysisThe detection ofgene rearrangenents indicatedwhether IgH (itnmunoglobulin heavy chain) or TcR-6 junctional regiotts should be analyzed by PCR (Polymerase chain reaction). The nucleotide sequence ofjunctional regions of the rearr...

Detection of Minimal Residual Disease in Acute Lymphoblastic Leukemia

MRD diagnostics during the first three months of treatment has proven to be of high value in pediatric acute lymphoblastic leukemia (ALL), because of its potential to recognize subgroups that differ substantially in outcome. Consequently, MRD diagnostics is now being used for treatment intervention, both treatment intensification (including stem cell transplantation) and treatment reduction. Ho...

Detection of minimal residual disease in acute lymphoblastic leukemia.

To detect more precisely the minimal residual disease in acute lymphoblastic leukemia (ALL), two-color flow cytometric analysis for the detection of cell-surface antigen (CD10; CALLA) and nuclear terminal deoxynucleotidyl transferase (TdT) was performed in the six patients with CALLA-positive ALL coexpressing TdT. In all patients, the leukemic blasts coexpressed Ia (HLA-DR), CD9, CD19, CD20, CD...